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Instrumentation

Simoa® HD-1 Analyzer

Quanterix's Simoa HD-1 Analyzer is a fully automated immunoassay platform with unprecedented ultra-sensitivity. Single molecule array (Simoa) testing can achieve quantitative biomarker analysis with sensitivity into the femtogram (fg/ml) range, representing up to 1,000 times higher sensitivity than traditional enzyme-linked immunosorbent assay (ELISA). 

How Simoa Technology Works

The main difference between Simoa and conventional immunoassays is in its ability to trap single molecules in femtoliter-sized wells, allowing for a digital readout.

This enhanced detection platform makes Simoa the premier choice for the quantitative detection of low-abundant and difficult-to-measure biomarkers where high sensitivity is an advantage.

  • Single protein molecules are captured and labeled on beads using standard ELISA reagents.
  • Tens of thousands of beads, with or without immunoconjugate, are mixed with enzyme substrate and loaded into individual femtoliter-sized wells that are engineered to house an individual bead.
  • The microwells are sealed with oil.
  • Fluorophore concentration in the small sample volume of wells containing the target analyte rapidly reach detectable limits using conventional fluorescence imaging which can be digitally counted.
  • The percentage of beads containing labeled immunocomplexes can be computed at low concentration because they follow a Poisson distribution: at higher concentrations, the intensity of the aggregate signal provides an analog measurement.
Sample requirement

Volume approximately 50–60 uL/assay for analysis of plasma, serum, central system fluid (cerebrospinal fluid, CSF), etc. Each assay must be carried out in duplicate and must achieve a %CV below 20 percent.

MESO QuickPlex SQ 120

The MESO QuickPlex SQ 120, a Meso Scale Discovery (MSD) platform, is the newest system from MSD. The MSD technology employs specific and accurate antibody arrays in a single well of a 96-well plate for maximizing available space for multiplexing and uses novel electrochemiluminescence for detection, which reduces background and enhances the dynamic range of the signals. 

This high-performance multiplexing makes MSD the premier choice for accurate measurements of up to 10 analytes at a time within the same sample.

How MSD Technology Works
Sample requirement

Volume approximately 10–50 uL/assay for analysis of plasma, serum, CSF, cell culture supernatant, cell lysate, etc. Each assay is carried out in duplicate and must achieve a %CV below 20%.

Have Questions or Need Help?

Contact us if you have questions or would like to learn more about the Proteomics & Metabolomics Core at Cedars-Sinai.