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Instrumentation

The core facility houses a variety of equipment for cloning, expression and purification of bacterially expressed recombinant proteins; this includes vectors for ligation-independent cloning (LIC), numerous strains of E. coli for expression of challenging constructs and a refrigerated incubator for large-scale production of temperature-sensitive proteins. For large-scale purification, the lab has a GE AKTA Purifier FPLC system equipped with a variety of columns for size exclusion, affinity and ion exchange chromatography.

A SensíQ® Pioneer SPR system is available for characterizing protein-ligand interactions. The machine has three channels and includes an autosampler and two temperature-controlled slots for SBS-footprint deep-well blocks. This instrument offers a significant advantage over the more frequently used GE BiaCore models as it can derive kinetic parameters from single "OneStep" injections.

Contact the Molecular Therapeutics Research Core

8700 Beverly Blvd.
Davis, 5007
Los Angeles, CA 90048